期刊论文

Wang, K.G.

中文

中国寄生虫学与寄生虫病杂志

1000-7423

2011

29

3

236-238

国家自然科学基金（No.30570952） 湖南省卫生厅项目（2009） 湖南省科技项目（2010）

本校为第一机构

利用双层琼脂平板法与荧光实时定量PCR(Real-time PCR)技术分别测定少量、中量和大量噬菌体的效价。结果显示,双层琼脂平板法与Real-time PCR法均可准确测定噬菌体效价。双层琼脂平板法所得符合条件的平板约为总数的1/3,需重复测定10次,每次消耗噬菌体10μl,测得噬菌体效价的组内变异系数为4.93%～30.38%。Real-timePCR法重复测定3次即可,每次消耗噬菌体1μl,其组内变异系数仅为0.02%～0.25%,明显低于双层琼脂平板法。提示,Real-time PCR技术可简单、迅速地测定噬菌体效价。

The phage titer of samples representing the low, intermediate and high phage number was respectively determined by the double-layer agar plate (DLAP) method and real-time PCR assay. The two methods accurately measured the titer of samples. The plaques from about 1/3 double-agar layer plates could be used to determine the phage titer. The DLAP experiment should repeat 10 times with 10 microl sample each time, while the within-assay coefficient variation (CV) was 4.93%-30.38%. At the same time, the real-time PCR assay only repeated 3 times with 1 microl phage each time, while CV for within-assay...