期刊论文

Analysis on human T cell epitopes polymorphisms of five specific antigens of Mycobacterium tuberculosis in 13 areas of China

中文

中华流行病学杂志

0254-6450

2016

37

4

553-557

10.3760/cma.j.issn.0254-6450.2016.04.023

国家科技重大专项（2013ZX10003006-002-001） 国家自然科学基金（81401647）

本校为第一机构

目的分析我国结核分枝杆菌复合群(MTBC)临床分离株中GlnA1、Mpt70、LppX、 GroES和LpqH5种抗原编码基因多态性及其人T细胞表位的多态性。方法选取13个省份临床分离的173株MTBC,采用PCR扩增5种抗原基因,并运用BioEdit软件进行序列比对,分析其人T细胞表位与非表位的变异情况。利用Mega 6.0软件分别计算同义突变率(dS)和非同义突变率(dN)及其比值。结果173株菌的基因glnA1非表位区出现2个非同义突变位点;基因mpl70表位区出现1个非同义突变位点;基因lpqH表位区表现为1个非同义突变位点和1个同义突变位点; groES在整个基因中未发现任何突变;基因lppX表位区表现为5个非同义突变和1个同义突变位点,其中...

OBJECTIVE: To investigate the polymorphisms of the coding gene and the human T cell epitopes of antigen GlnA1, Mpt70, LppX, GroES and LpqH on Mycobacterium tuberculosis complex (MTBC) strains in thirteen provinces of China. METHODS: A total of 173 clinical MTBC isolates from thirteen provinces were selected to test the gene sequences of the five antigens, using PCR and DNA sequencing methods. Sequences were compared and sliced by BioEdit, and the variations of the human and nonhuman T cell epitopes were analyzed. The rates on synonymous mutation (dS), non-synonymous mutation (dN) and dN/dS val...