HUH endonuclease-mediated DNA-protein conjugates: sequence-specific tools and cellular applications

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成果类型：

期刊论文

作者：

Huang, Xiaowei;Li, Haokun;Du, Jiajun;Xie, Wenxue;Liu, Yuanyuan;...

通讯作者：

Xing, H

作者机构：

[Huang, Xiaowei; Lin, Yingwu] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.

[Xie, Wenxue; Huang, Xiaowei; Du, Jiajun; Li, Haokun; Xing, Hang] Hunan Univ, Inst Chem Biol & Nanomed, State Key Lab Chemo & Biosensing, Coll Chem & Chem Engn,Hunan Prov Key Lab Biomacrom, Changsha 410082, Peoples R China.

[Liu, Yuanyuan] Hunan Inst Drug Control, Changsha 410001, Hunan, Peoples R China.

[Xing, Hang] Hunan Univ Chongqing, Res Inst, Chongqing 401100, Peoples R China.

通讯机构：

[Xing, H ] H

Hunan Univ, Inst Chem Biol & Nanomed, State Key Lab Chemo & Biosensing, Coll Chem & Chem Engn,Hunan Prov Key Lab Biomacrom, Changsha 410082, Peoples R China.

Hunan Univ Chongqing, Res Inst, Chongqing 401100, Peoples R China.

语种：

英文

期刊：

Chemical Communications

ISSN：

1359-7345

年：

2025

卷：

期：

页码：

16498-16511

DOI：

10.1039/d5cc04628a

基金类别：

National Natural Science Foundation of China [2024YFA1209401]; National Key Research and Development Program of China [22374043]; National Natural Science Foundation of China [2022SK2003]; Hunan Provincial Science and Technology Department [2022RC3046]; Science and Technology Innovation Program of Hunan Province; Natural Science Foundation of Chongqing

机构署名：

本校为第一机构

院系归属：

化学化工学院

摘要：

This highlight review article summarizes recent advances in employing HUH endonucleases as self-labeling protein tags for the sequence-specific covalent conjugation of unmodified ssDNA and examines their applications in cellular studies via engineered DNA-protein conjugates. We outline the structural basis and catalytic mechanism of the conserved HUH and Y motifs, which enable high selectivity, bioorthogonality, and robust conjugation under physiological conditions. Recent applications demonstrate the versatility of HUH-based DNA-protein conjugates in programmable cellular interface engineerin...

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HUH endonuclease-mediated DNA-protein conjugates: sequence-specific tools and cellular applications

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