期刊论文

Detection of Radon Using a Label-free Fluorescence Sensor Based on Combination of Pb~(2+) -induced Allosteric T30695 with N-Methyl Mesoporphyrin Ⅸ

中文

分析测试学报

1004-4957

2019

38

6

643-648

10.3969/j.issn.1004-4957.2019.06.002

国家自然科学基金资助项目（81473021）；

本校为第一机构

氡衰变形成稳定的子体铅只需约3. 82 d,且衰变产生的~(210) Pb非常稳定,其半衰期为21年。基于氡辐射衰变最终形成稳定的子体铅的特点,探讨了子体铅诱导富鸟嘌呤核苷酸序列形成G-四链体的特性,建立了一种基于子体铅诱导T30695构象改变的荧光传感器。氡的子体铅诱导T30695形成的G-四链体结构能与N-甲基卟啉二丙酸Ⅸ(NMM)荧光染料结合发出较强荧光,在一定范围内,荧光强度与Pb~(2+)的浓度和氡累积辐射剂量浓度成正相关。Pb~(2+)离子浓度在5. 13 × 10~(-9)~ 1. 25 × 10 ~(- 7)mol/L范围内与体系的ΔIF值呈现良好的线性关系,回归方程为ΔI_F = 77. 30 + 5. 05c,r = 0. 995 0,对铅的检出限为1. 54 ×...

A fluorescent sensor was fabricated for the detection of radon based on the daughter lead inducing G-rich aptamer T30695 to form G-quadruplex. According to the characteristics of radon radioactive decay,it only takes about 3. 82 days for radon to decay and form a stable daughter lead, and ~(210)Pb derived from radon decay is very stable. The half-life period of the stable daughter(~(210)Pb) resulted from radon is 21 years,and the lead daughter product could be used as the target for detection. When there was no lead in the system,T30695 combine...