期刊论文

中文

分析测试学报

1004-4957

2007

26

2

216-220

10.3969/j.issn.1004-4957.2007.02.017

湖南省自然科学基金（03JJY3030）；

本校为第一机构

基于乙基紫(EV)与1-羟基芘(1-OHP)反应形成离子缔合物,导致体系的共振光散射(RLS)增强,建立了测定1-OHP的共振光散射法.在pH 8.0Tris-HCl缓冲介质中,最大RLS峰位于396 nm,其强度与1-OHP浓度成线性关系.加入表面活性剂十二烷基苯磺酸钠(SDBS),体系的灵敏度可提高两倍以上.方法的线性范围为4.0～982 μg·L~(-1),相关系数r=0.999 4,检出限1.2 μg·L-1,相对标准偏差为5.8%～7.9%,平均回收率为94%(n=6).已成功用于人尿中痕量1-OHP的测定,结果满意.

A novel method for the trace determination of 1-hydroxypyrene （1-OHP） has been developed. It was based on the enhancement of resonance light scattering（RLS） of the ion-associate complex formed between ethyl violet（EV） and 1-OHP. In Tris- HCl buffer solution of pH 8.0, the maximum RLS peak was located at 396 nm and the enhanced RLS intensity at this wavelength was directly proportional to the 1-OHP concentration. The sensitivity of 1-OHP -EV complex in the presence of sodium dodecylbenzene sulfonate （SDBS） was twice as high as that in th...