期刊论文

中文

分析测试学报

1004-4957

2004

23

5

56-59

10.3969/j.issn.1004-4957.2004.05.013

湖南省卫生厅科研资助项目（Y02-070）；

本校为第一机构

通过研究F-离子对人血清白蛋白(HSA)溶液共振Rayleigh光散射(RLS)的增强效应,建立一种基于生物蛋白RLS增强测定环境中氟离子的新型检测方法.实验条件为λex=λem=368 nm,pH=5.80,t=32℃.结果表明,应用含氟离子溶液与空白溶液在368nm处RLS差值(△I),标准曲线法测定样品中的氟离子,方法线性范围为4.78～16.2 μg/mL,r=0.992,检出限为1.43μg/mL.方法精密度(RSD为1.31%～5.72%)和准确度(回收率R为91%～109%)较好.

A novel method for the determination of fluoride ion(F - )with human serum albumin(HSA)was developed.It was based on the enhancement effect of F - on the resonance Rayleigh light scattering(RLS)of HSA in K 2 HPO 4 -NaH 2 PO 4 buffer solution.It was found that the enhanced intensity of RLS(ΔI)was proportional to the concentration of F - under the selected conditions:λ ex =λ em =368nm,pH=5.80,temperature=32℃,lay down time=30min.The linear range of the method was4.78～ 16.2μg/mLwith a correlation coefficient of0.992.The detection limit was1.43μg/mL.The RSD and the r...