期刊论文

Liu, Ji-Fang

英文

世界胃肠病学杂志：英文版

1007-9327

2011

17

32

3700-3708

10.3748/wjg.v17.i32.3700

Supported by Natural Science Foundation of Hunan Province No.09JJ3060 Health Bureau Fund of Guangzhou No.201102A213006 Education Bureau Fund of Guangzhou No.10A186 We thank Lu MY for kindly technical assistance and Qi YC for comments on the manuscript.

本校为其他机构

AIM: To explore the effects of siRNA silencing of PIK3CA on proliferation, migration and invasion of gastric cancer cells and to investigate the underlying mechanisms. METHODS: The mutation of PIK3CA in exons 9 and 20 of gastric cancer cell lines HGC-27, SGC-7901, BGC-823, MGC-803 and MKN-45 was screened by poly-merase chain reaction (PCR) followed by sequencing. BGC-823 cells harboring no mutations in either of the exons, and HGC-27 cells containing PIK3CA mutations were employed in the current study. siRNA targeting PIK3CA was chemically synt...

瞄准：在胃的癌症房间的增长，移植和侵略上探索 PIK3CA 的 siRNA silencing 的效果并且调查内在的机制。方法：在 exons 的 PIK3CA 的变化 9 胃的癌症和 20 房间线 HGC-27， SGC-7901， BGC-823， MGC-803 和 MKN-45 被定序跟随的聚合酶链反应(PCR ) 屏蔽。不 exons 在也怀有变化的 BGC-823 房间，和包含 PIK3CA 变化的 HGC-27 房间在当前的学习被采用。指向 PIK3CA 的 siRNA 化学上被综合并且是进在 vitro 的这二根房间线的 transfected。mRNA 和 PIK3CA 的蛋白质表示被即时 PCR 并且分别地的西方的弄污检测。我们也用西方的弄污测量了 serine/threonine 蛋白质 kinase (Akt ) 的 phosphoryla...