期刊论文

Su, Qi

英文

世界胃肠病学杂志：英文版

1007-9327

2004

10

18

2731-2734

10.3748/wjg.v10.i18.2731

Supported by the Natural Science Foundation of Hunan Province,No.02JJY2026,01JJY2146 and the Foundation of Hunan ProvinceEducation Department,No.01A016；

本校为通讯机构

Aim: To determine the role of p38 MAP kinase signal transduction pathways in diallyl disulfide (DADS)-induced G2/M arrest in human gastric cancer MGC803 cells. Methods: MGC803 cell growth inhibition was measured by MTT assay. Phase distribution of cell cycle was analyzed by flow cytometry. Expression of Cdc25C, p38, phosphorylation of p38 (pp38) were determined by Western blotting. Results: MTT assay showed that SB203580, a specific p38 MAPK inhibitor blocked DADS-induced growth inhibition. Flow cytometry analysis revealed that treatment of MGC...

AIM:To determine the role of p38 MAP kinase signaltransduction pathways in diallyl disulfide （DADS）-inducedG2/M arrest in human gastric cancer MGC803 cells.METHODS:MGC803 cell growth inhibition was measuredby MTT assay.Phase distribution of cell cyde was analyzed byflow cytometry.Expression of Cdc25C,p38,phosphorylationof p38 （pp38） were determined by Western blotting.RESULTS:MTT assay showed that SB203580,a specificp38 MAPK inhibitor blocked DADS-induced growth inhibition.Flow cytometry analysis revealed that treatment of MGC803cells with...