期刊论文

中文

北京大学学报(医学版)

1671-167X

1999

31

5

404-407

10.3321/j.issn:1671-167X.1999.05.006

国家自然科学基金

本校为其他机构

目的:探讨内源性血红素加氧酶(heme oxygenase, HO)/一氧化碳(carbon monoxide, CO)系统对大鼠主动脉球囊损伤后新生内膜形成及丝裂素活化蛋白激酶(mitogen-activated protein kinase, MAPK)激活的调节作用.方法:采用大鼠主动脉内膜球囊拉伤模型,观察HO抑制剂锌卟啉-9(zinc protoporphyrin IX, ZnPP-9)或其底物血红素-左旋-赖氨酸盐(heme-L-lysinate, HLL)对血管壁细胞3H-TdR掺入和MAPK活性的影响,同时观察血管平滑肌HO活性和CO生成的变化.结果:内膜拉伤后2周血管3H-TdR掺入和MAPK活性明显增加,同时HO活性和CO的产生增加;ZnPP-9使血管的3H-TdR掺入和MAPK活性增加更为明显(分别比单独拉伤组增加3...

Objective: The purpose of this study was to investigate the role of endogenous carbon monoxide (CO) on neointimal formation and mitogen activated protein kinase (MAPK) activity. Methods: On a rat model of aortic endothelium balloon injury. Vascular 3H TdR incorporation, MAPK activity, heme oxygenase (HO) activity and CO release were measured after treatment of ZnPP 9, an HO inhibitor, or heme L lysinate (HLL), an HO substrate. Results: Vascular 3H TdR incorporation and MAPK activity were significantly increased on day 14 after endothelium balloon injury while HO activity and CO release were up...