期刊论文

Xie, Shuibo(xiesbmr@263.net)

中文

哈尔滨工业大学学报

0367-6234

2016

48

8

115-120

10.11918/j.issn.0367-6234.2016.08.019

51408293:国家自然科学基金 2014M562114:中国博士后科学基金面上项目 14B154:湖南省教育厅优秀青年项目

本校为第一且通讯机构

为揭示柠檬酸废水生物处理过程中功能菌群作用机制,以柠檬酸工业废水内循环厌氧反应塔(IC)中厌氧颗粒污泥为研究对象,统计颗粒粒径分布,通过环境扫描电子显微镜(ESEM)观察颗粒微观形态结构,利用高通量测序技术分析微生物多样性及菌群特征.结果发现,粒径在1.0~4.0 mm的颗粒所占比例最多,为74.4%.ESEM显示微生物分布以球形细菌为主.高通量测序得到8 397条有效序列,可划分操作分类单元(OTU)873个,Alpha多样性指数显示样品文库覆盖率0.936,Shannon指数为4.376,而ACE指数与Chao1指数分别为3 415.51与2 246.51,反映颗粒污泥中微生物种类与数量均较多.微生物菌群主要包括4大类,分别为可降解有机物的水解...

To clarify the performance of functional microorganisms involved in biological citric acid wastewater treatment systems, bacterial community structure of anaerobic granular sludge was investigated in an internal circulation (IC) anaerobic reactor treating citric acid industry wastewater. Microstructure observation was carried out by environmental scanning electron microscope (ESEM), and the microbial diversity and microfloras were analyzed by high-throughput sequencing. Particle size distribution results showed that the most particles' diameter...