Iron is crucial for numerous biological processes, and lysosomes play an essential role in iron metabolism by regulating Fe 3+ levels. Disruptions of this regulation can lead to Fe 3+ accumulation, resulting in membrane damage and ferroptosis. Here, we have developed a water-soluble fluorescent probe BiNIT that specifically targets lysosomes for the selective detection of Fe 3+ . BiNIT features a bis-naphthalimide structure linked by a thiophene moiety and incorporates two quaternary ammonium groups, which enhance its ability to target lysosomes and its solubility in aqueous environments. The ...
