期刊论文

中文

中国病毒学：英文版

1674-0769

2005

20

5

464-467

湖南省教育厅非典科技攻关项目(0309)

本校为第一机构

本研究根据GenBank登录的BJ01株SARS-CoV序列合成801bpS1基因片段,该片段被亚克隆至真核表达载体pcDNA3.1(+)得到重组质粒pcDNA3.1(+)/S1;转染Hela细胞,SDS-PAGE、Western-Blotting鉴定蛋白表达;肌注免疫BALB/c小鼠,利用ELISA法检测免疫后小鼠的抗SARS/CoVIgG及IFN-γ水平,MTT法检测T细胞增殖活性.结果显示,重组质粒pcDNA3.1(+)/S1可在Hela细胞内表达S1蛋白,免疫后小鼠的T细胞增殖活性增强,抗SARS-CoVIgG与IFN-γ水平升高.本实验说明pcDNA3.1(+)/S1可诱导小鼠产生一定的体液免疫和细胞免疫应答.

The 801 base pairs of S1 gene fragment were synthesized based on the Sever acute respiratory syndrome associated coronavirus（SARS-CoV）BJ01 strain registered in GenBank. The synthetical DNA was subcloned into the appropriate site of pcDNA3.1 （＋） eukariotic expression vector to construct a recombinant plasmid pcDNA3.1（＋）/S1;The recombinant plasmid pcDNA3.1（＋）/S1 was transfected into Hela cells and the expressed protein was identified By SDS-PAGE and Western-blotting;BALB/c mice were immunized with pcDNA3.1（＋）/S1 by i. m. The level o...