期刊论文

中文

中国病毒学：英文版

1674-0769

2002

17

3

230-233

教育部高校骨干教师资助计划

本校为第一机构

为了探讨腺病毒(adenovirus,Ad)E1B 55kD癌蛋白(Ad E1B 55kD)打破hDaxx和PML共定 位细胞核的作用机制,本文利用体内外共免疫沉淀反应研究Ad E1B 55kD与hDaxx的结合反应 ,并通过酵母双杂交体系测定两种蛋白质的相互作用及其作用的氨基酸残基序列.结果显示 :Ad2 E1B 55kD通过C端58个氨基酸(aa)与hDaxx结合并发生相互作用.Ad12 E1B 5 5kD与hDaxx结合需全序列aa及其构象.共免疫沉淀反应和Western blot结果证实Ad2/5或Ad1 2 E1B 55kD能在体内外与hDaxx直接结合.

To question the reason of co localization of hDaxx and PML in PODs disrupted by Adenovirus(Ad) E1B 55 kilodalton oncoproteins(Ad E1B 55kD), the interaction of Ad E1B 55kD with hDaxx was analyzed by the yeast two hybrid assay, and the direct binding of Ad E1B 55kD with hDaxx was studied by coimmunoprecipitation reaction \%in vivo\% or \%in vitro\%. The results show that Ad E1B 55kD oncoproteins interact with hDaxx by binding the C terminus 58 amino acids of Ad2 E1B 55kD or the full length of Ad12 E1B 55kD. Ad2/5 or Ad1...