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The Third Generation of Artificial Dye-Decolorizing Peroxidase Rationally Designed in Myoglobin

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成果类型:
期刊论文
作者:
Zhang, Ping;Xu, Jiakun;Wang, Xiao-Juan;He, Bo;Gao, Shu-Qin;...
通讯作者:
Lin, Ying-Wu
作者机构:
[Lin, Ying-Wu; Wang, Xiao-Juan; Zhang, Ping; He, Bo] Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
[Xu, Jiakun] Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fisheries, Qingdao 266071, Shandong, Peoples R China.
[Xu, Jiakun] Lab Marine Drugs & Byprod Pilot Natl Lab Marine S, Qingdao 266071, Shandong, Peoples R China.
[Gao, Shu-Qin; Lin, Ying-Wu] Univ South China, Tab Prot Struct & Funct, Hengyang 421001, Peoples R China.
通讯机构:
[Lin, Ying-Wu] U
Univ South China, Sch Chem & Chem Engn, Hengyang 421001, Peoples R China.
Univ South China, Tab Prot Struct & Funct, Hengyang 421001, Peoples R China.
语种:
英文
关键词:
dye-decolorizing peroxidases;artificial enzyme;protein design;myoglobin;substrate-binding;molecular docking
期刊:
ACS CATALYSIS
ISSN:
2155-5435
年:
2019
卷:
9
期:
9
页码:
7888-7893
基金类别:
It is a pleasure to acknowledge Prof. S. G. Sligar and Prof. Y. Lu of University of Illinois at Urbana–Champaign, for the kind gift of the sperm whale Mb gene. This work was supported by the National Natural Science Foundation of China (31370812, 21807058, and 31200642), Special Scientific Research Funds for Central Nonprofit Institutes, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences (20603022016011), and Financial Fund of the ministry of Agriculture and Rural Affairs, P. R. of China (NFZX2018).
机构署名:
本校为第一且通讯机构
院系归属:
化学化工学院
摘要:
Approaches to degradation of industrial dyes are desirable, of which bioremediation is more favorable. In addition to the use of native enzymes, rational design of artificial enzymes provides an alternative approach. Meanwhile, few designs can achieve a catalytic activity comparable to that of native enzymes. We have previously designed two generations of artificial dye-decolorizing peroxidases (DyPs) in myoglobin (Mb) by introduction of Tyr43 and Trp138 in the heme pocket; however, the activity is moderate. To improve the activity of the artif...

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