期刊论文

Protective mechanism of ischemic preconditioning on apoptosis induced by kidney ischemia-reperfusion

中文

中华实用儿科临床杂志

2095-428X

2016

31

5

354-358

10.3760/cma.j.issn.2095-428X.2016.05.009

(2014SK2008):湖南省科技厅重点科研基金 (2014SK2008):湖南省***卫生人才“225”工程资助Fund programKey Scientific Research Project of Hunan Provincial &Technology (＂225＂ Project):High - Level Medical Personnel of Hunan Province

本校为第一机构

目的探讨缺血预处理(IPC)对大鼠缺血再灌注(IR)肾损伤引起肾小管上皮细胞凋亡的保护作用及其与Bcl-2、Bax表达变化的关系。方法采用夹闭小鼠双侧肾蒂30 min的方法建立小鼠IR肾损伤模型;微型动脉夹夹闭小鼠双侧肾蒂15 min的方法进行预缺血,4 d后再进行IR。实验共分为5个大组:对照组(C组),假手术组(S组),IR组,IPC组(IPC+IR组),IPC对照组(S+IR组);除C组外,其他4组根据再灌注的时间分亚组(再灌注后0 h,3 h,6 h,12 h,24 h,48 h,3 d,5 d,7 d);反转录聚合酶链反应(RT-PCR)方法检测小鼠肾组织Bax、Bcl-2 mRNA表达水平;免疫组织化学方法检测小鼠肾组织Bax、Bcl-2的分布及蛋白质表达水平。原位末端转移酶标...

Objective To investigate the protective effect of ischemia preconditioning(IPC)on apoptosis in-duced by renal ischemia - reperfusion(IR)and relations to the changing expressions of Bcl - 2,Bax in rat kidney. Methods Ischemia models were induced by clipping bilateral renal pedicles for 30 min by using the artery clamp;IPC group was induced by clipping bilateral renal pedicles for 15 min,4 days later IR was performed again by clipping bila-teral renal pedicle for 30 min. Rats were randomly divided into 5 groups with 5 animals in each group:contro...