期刊论文

中文

中华实用儿科临床杂志

2095-428X

2006

21

1

14-16

10.3969/j.issn.1003-515X.2006.01.007

国家自然科学基金项目资助（30271665）；

本校为第一机构

目的探讨黄芪活性成分黄芪甲甙对病毒性心肌炎（VM）小鼠心肌中神经生长因子（NGF）表达的影响。方法Balb/c小鼠100只,随机分成6组。非感染小鼠腹腔无菌注射病毒培养液,分为正常对照组[A组,10只,以羧甲基纤维素钠（CMC）0.1 mL灌胃7 d]、高剂量对照组（B组,10只,9%黄芪甲甙液0.1 mL灌胃7 d）;余80只小鼠以柯萨奇病毒（CVB3）腹腔无菌注射制作VM模型,VM小鼠随机分为心肌炎对照组和低、中、高剂量干预组,分别以CMC、1%、3%、9%黄芪甲甙液0.1 mL灌胃7 d（分别为C、D、E、F组,每组20只）。14 d后处死小鼠并取其心脏,采用RT-PCR和免疫组织化学检测NGF mRNA及蛋白表达水平。结果F组小鼠较C组死亡率明显...

Objective To investigate the effect of astragaloside, one of the active components of astragalus membranaeeus, on myocardial nerve growth factor（NGF） expression in murine myocarditis model with eoxsackievirus B （CVB3）. Methods One hundred Balb/c mice were randomly divided into 6 groups. （;roup A [ n = 10, treated with carboxymethylcellulose（CMC） 0.1 mL, ig, for 1 week] were served as normal controls group and group B （ n - 10, treated with 9 % astragaloside 0. 1 mL, ig, for 1 week）as high - dose control. Eighty infected mice treated wi...