Development of cross‐priming amplification assays for rapid and sensitive detection of Aeromonas hydrophila

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Meng, S.;Wang, Y.;Liu, D.;Ye, C.*

通讯作者：

Ye, C.

作者机构：

[Meng, S.; Ye, C.; Wang, Y.] China CDC, State Key Lab Infect Dis Prevent & Control, Natl Inst Communicable Dis Control & Prevent, Beijing 102206, Peoples R China.

[Meng, S.; Ye, C.; Wang, Y.] Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China.

[Liu, D.] Univ South China, Pathogen Biol Inst, Hengyang, Hunan, Peoples R China.

[Ye, C.] China CDC, State Key Lab Infect Dis Prevent & Control, Natl Inst Communicable Dis Control & Prevent, Changbai Rd 155, Beijing 102206, Peoples R China.

通讯机构：

[Ye, C.] C

China CDC, State Key Lab Infect Dis Prevent & Control, Natl Inst Communicable Dis Control & Prevent, Changbai Rd 155, Beijing 102206, Peoples R China.

语种：

英文

关键词：

Aeromonas hydrophila;cross-priming amplification;desA gene amplification;detection;quantitative PCR

期刊：

Letters in Applied Microbiology

ISSN：

0266-8254

年：

2015

卷：

期：

页码：

171-178

DOI：

10.1111/lam.12439

基金类别：

Mega Project for Research on The Prevention and Control of HIV/AIDS, Viral Hepatitis Infectious Diseases of the Ministry of Science and Technology, People's Republic of China [2011ZX10004-001, 2013ZX10004-101]

机构署名：

本校为其他机构

院系归属：

医学院

摘要：

UNLABELLED: Aeromonas hydrophila has been increasingly implicated as the aetiologic agent of various human diseases. Therefore, reliable laboratory detection and identification of this bacterium has become clinically and epidemiologically desirable. We developed a nearly instrument-free, simple molecular method for rapid detection of Aer. hydrophila using a cross-priming amplification (CPA) assay with the desA gene as the target. The desA gene is crucial for the survival and growth of Aer. hydrophila under iron starvation. The results can be visualized as colour changes without opening the rea...

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Development of cross‐priming amplification assays for rapid and sensitive detection of Aeromonas hydrophila

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