期刊论文

Xi, S.

中文

中华实验眼科杂志

2095-0160

2005

23

1

4-7

10.3760/cma.j.issn.2095-0160.2005.01.002

国家自然科学基金 （30370675 ）； 日本大塚制药厂新药研究基金（2001 8 28）资助；

本校为第一机构

目的观察血管内皮生长因子(VEGF)在早期糖尿病视网膜病变中的表达以及Ibrolipim(N0-1886)对2型糖尿病小型猪血糖的影响;探讨药物Ibrolipim对视网膜VEGF的作用.方法采用高脂高蔗糖饲料喂养贵州小香猪,创建2型糖尿病动物模型,并用Ibrolipim进行干预,用RT-PCR、免疫组织化学和Western blot技术检测VEGF转录和蛋白表达.结果正常组视网膜VEGFmRNA表达量少,免疫组织化学反应阳性较弱,VEGF蛋白表达较低;实验组视网膜VEGFmRNA表达上调,免疫组织化学反应呈强阳性(P＜0.01),VEGF蛋白表达增高;治疗组视网膜VEGFmRNA及VEGF蛋白表达介于其他两组之间.结论早期糖尿病视网膜中VEGF表达增高;Ibrolipim可通过降低...

Objective: To observe the correlation of Ibrolipim (NO-1886) with expression of vascular endothelial growth factor (VEGF) in early diabetic retinopathy in minipigs. Methods: Fifteen minipigs were assigned to 3 groups according their weight. The minipigs in the normal control group were fed with general diet and high glucose diet for experimental group, and high fat/high sucrose diet was gave for 3 months and then Ibrolipim were fet in treatment group. The level of VEGF in retina was measured by RT-PCR, immunohistochemistry and Western blot 8 months later. Results: A smaller amount of positive ...