期刊论文

Mouse hepatocarcinomas with different metastatic potential have different effect on lymphatic system of host

中文

实用肿瘤杂志

JOURNAL OF PRACTICAL ONCOLOGY

0258-0926

2000

36

2

90-92

10.3969/j.issn.1001-1692.2000.02.006

国家自然科学基金! (39470 776 )；

本校为其他机构

目的　研究高低转移力小鼠肝癌细胞 HCa- F和 HCa- P对宿主淋巴系统的影响及其机制。方法　以流式细胞仪动态检测皮下接种 F或 P细胞后的 6 15小鼠局部引流淋巴结内淋巴细胞的细胞周期。以免疫组化法检测F和 P细胞表达 Fas L 的情况 ,以 TU NEL 法观察转移淋巴结内巨噬细胞的凋亡情况。结果　 F组小鼠引流淋巴结内淋巴细胞增殖峰出现晚于 P组 ,且持续时间短 ,迅速滑落至低位。F细胞表达 Fas L 强于 P细胞。F细胞周围的巨噬细胞发生凋亡。结论　宿主对 F细胞的免疫应答弱于 P。这可能与 F细胞表达较多 Fas L 诱导巨噬细胞凋亡 ,从而抑制抗原提呈作用有关。

Objective To investigate the effect of mouse hepatocarcinoma cell line HCa F(high metastatic potential)and HCa P(low one) on lymphatic system of host.Methods Mice were subcutaneously implanted with HCa F or HCa P cells;cell cycle of lymphocytes from tumour bearing mice was studied by flow cytometry.FasL expressions of F and P cells were detected immunohistochemically.Apoptosis of macrophages in metastatic lymph nodes was examined by TUNEL method.Results The proliferation of lymphocytes in mice with F cells was much stronger than those with P cells.The proliferative peak of lymphacytes in mice ...