The nucleus and cytoplasm margin of a single cervical cell are extracted precisely. In order to obtain the edge of cell image by using GVF Snake model,firstly it is required to get initial contour. For the cytoplasm,the initial contour is obtained by Otsu method. For the nucleus,the Otsu method can not be segmented correctly due to the fact that the relative size between the divided object and the background is too large. Therefore,the initial contour of the cell nucleus is extracted by using the improved global threshold method with gradient edge information. By placing an accurate initial co...