期刊论文

中文

中国免疫学杂志

1000-484X

2022

38

13

1585-1589

10.3969/j.issn.1000-484X.2022.13.009

湖南省自然科学基金项目（2021JJ30606,2022JJ60104）； 湖南省卫生健康委员会项目（202102060874）；

本校为第一机构

目的:探讨异荭草素对盲肠穿刺法(CLP)诱导的脓毒症小鼠急性肺功能损伤的影响。方法:将健康小鼠随机分为5组:假手术对照组、模型组及异荭草素低(7.5 mg/kg)、中(15 mg/kg)、高(30 mg/kg)剂量组,除假手术组开腹不穿刺外,其余各组采用CLP建立脓毒症模型,各组于术后2 h给药,共7 d,末次给药后,检测小鼠肺组织干湿重比及肺功能指标(静息通气量、气道阻力、肺容积);HE染色观察肺组织;ELISA检测血清及肺组织炎症指标水平(iNOS 、IL-10);Western blot检测肺组织细胞凋亡相关蛋白表达量(cleaved caspase 9/caspase 9、cleaved caspase 3/caspase 3);Masson染色观察肺纤维化;Western blot检测肺组织中NF-κ...

Objective:To investigate the effect of isoorientin on acute lung injury and in mice with sepsis induced by cecal ligation and puncture(CLP).Methods:Healthy mice were randomly divided into five groups:sham operation control group,model group and isoorientin low(7.5 mg/kg),medium(15 mg/kg)and high(30 mg/kg)dose groups.Except for the sham operation group,the CLP method was used to establish a sepsis model.Each group was administered 2 h after surgery for a total of 7 d.After the last administration,the dry and wet lung tissues.Comparative lung fun...