期刊论文

中文

中国免疫学杂志

1000-484X

2022

38

5

545-550

10.3969/j.issn.1000-484X.2022.05.007

湖南省自然科学基金项目（2018JJ6122）；

本校为其他机构

目的:研究miR-95-5p对非小细胞肺癌(NSCLC)细胞增殖和侵袭的作用及机制。方法:RT-qPCR检测NSCLC组织和细胞中miR-95-5p与SATB1 mRNA的表达,利用Lipofectamine2000将miR-NC、miR-95-5p mimic、pc-NC、pc-SATB1质粒分别或联合转染至H1650和A549细胞,CCK-8实验检测细胞增殖,Transwell实验检测细胞侵袭,双荧光素酶报告检测miR-95-5p与SATB1靶向关系,Western blot检测SATB1蛋白相对表达水平。结果:miR-95-5p在NSCLC组织和细胞中均明显下调(P

Objective:To study the effect and mechanism of miR-95-5p on the proliferation and invasion of non-small cell lung cancer(NSCLC)cells.Methods:The expression of miR-95-5p and SATB1 mRNA in NSCLC tissues and cells was detected by RTqPCR.Lipofectamine 2000 was used to transfect miR-NC,miR-95-5p mimic,PC-NC and PC-SATB1 plasmids into H1650 and A549 cells,respectively or in combination.Cell proliferation was detected by CCK-8 experiment,cell invasion was detected by Transwell experiment.Targeting relationship of miR-95-5p and SATB1 was detected by du...