期刊论文

中文

中国免疫学杂志

1000-484X

2022

38

10

1177-1182

10.3969/j.issn.1000-484X.2022.10.005

湖南省自然科学基金项目（2022JJ60104,2022JJ70039）；

本校为第一机构

目的:研究氯化两面针碱(NC)对肺癌细胞生长、运动及炎症因子的影响,并阐明其可能机制。方法:以肺癌细胞系A549和NCI-H661为体外研究模型,CCK-8测定不同浓度NC对细胞活力的影响。选择0、3、6、12 nmol/L 4个浓度处理细胞,EdU染色测定细胞增殖,Transwell测定细胞侵袭,微管形成实验测定细胞微管形成能力,RT-PCR检测炎症因子iNOS、 TNF-α、IL-4和IL-10 mRNA表达,ELISA检测iNOS、TNF-α、IL-4和IL-10含量,Western blot检测VEGF、N-cadherin和E-cadherin蛋白水平及AKT/ERK1/2通路磷酸化。加入AKT激活剂SC79(4 μg/ml)进行验证实验。12周龄雄性BALB/c小鼠作为体外研究对象,检测NC对肿瘤的影响。结果:与...

Objective:To study the effects of Nitidine chloride(NC)on lung cancer cell growth,motility and inflammatory factors,and to clarify its possible mechanisms.Methods:The lung cancer cell lines A549 and NCI-H661 were used as research models in vitro.CCK-8 was used to determine the effects of different concentrations of NC on cell viability.Four concentrations of 0,3,6,and 12 nmol/L were selected to treat cells,EdU staining was used to measure cell prolferation,Transwell were used to measure cell invasion,microtubule formation experiment were used t...