期刊论文

Immuno-suppressive function of myeloid derived suppressor cells to T cells in BALB/c mice infected with Schistosoma japonicum

中文

中国免疫学杂志

1000-484X

2019

35

3

269-273

10.3969/j.issn.1000-484X.2019.03.003

湖南省教育厅科学研究项目(No.13C824) 国家自然科学基金(No.81101274)资助。

本校为第一机构

目的:研究日本血吸虫感染小鼠模型中的髓源抑制细胞(MDSCs)的免疫抑制功能及对T细胞的作用机制。方法:构建日本血吸虫感染BALB/c小鼠模型,流式细胞术检测MDSCs动态比例变化,免疫磁珠分选小鼠骨髓中单核系MDSCs(CD11b~+Gr1~+Ly6G~-)和粒系MDSCs(CD11b~+Gr1~+Ly6G~+)亚群细胞,Write-Giemsa染色进行形态学鉴定;CFSE法检测单核系和粒系MDSCs亚群对CD4~+ T、CD8~+ T细胞增殖的抑制作用,Real-time PCR方法检测细胞因子IFN-γ、IL-4、IL-10、IL-13、TGF-β和精氨酸酶(ArgⅠ)、一氧化氮合酶2(NOS2)的表达。结果:血吸虫感染模型小鼠的MDSCs较对照组小鼠明显增多,以粒系MDSCs(CD11b~+Gr1~+Ly6G~+)亚群增多...

Objective:To study the morphology and immunosuppressive function of myeloid-derived suppressor cells(MDSCs)and its subsets,and the mechanism of immunosuppressive function to T cells in mice infected with Schistosoma japonicum.Methods:The BALB/c mice model of Schistosoma japonicum infection were construted,and the dynamic changes of MDSCs ratio were detected by flow cytometry.The MDSCs were isolated by immunomagnetic separation technology to monocytic cells(CD11b~+ Gr1~+ Ly6G~-)and granulocyte cells(CD11b~+Gr1~+Ly6G~+),and dyed by Write-Giemsa s...