期刊论文

Proteomics screen Chlamydia trchomatis immunodominant infection dependant antigens

中文

中国免疫学杂志

1000-484X

2018

34

11

1684-1689

10.3969/j.issn.1000-484X.2018.11.017

No.81471969、No.81202374;No.31600150:国家自然科学基金 No.2015-351:湖南省分子靶标新药研究协同创新中心资助项目 NO.2014-5:特殊病原体防控湖南省重点实验室项目 :湖南省大学生研究性学习和创新性实验计划

本校为第一机构

目的:采用蛋白质组学方法筛选沙眼衣原体(Chlamydia trachomatis,Ct)感染依赖性免疫优势抗原。方法:将纯化的Ct D型标准株EB经紫外线照射制备UV-EB,然后将未经处理的活EB和紫外线处理的UV-EB分别感染体外培养的HeLa细胞,间接免疫荧光法检测紫外线对EB的灭活效果;分别将低剂量活EB滴鼻感染小鼠或将较高剂量UV-EB经肌肉接种免疫小鼠,首先检测两种接种方式下Ct在体内的增殖情况,然后收集两组小鼠的血清,分别与Ct D型全基因组908个开放读码框编码的GST-Ct融合蛋白进行ELISA反应,蛋白质组学筛选仅与活EB感染组小鼠血清反应且反应频率高的Ct感染依赖性免疫优势抗原;最后用筛选到的抗原体外刺激Ct生殖道...

Objective:To screen for Chlamydia trachomatis immunodominant infection dependent antigens by using proteome array assay.Methods:C.trachomatis serovar D UW-3/CX strain EBs were inactivated by exposure of EB preps to UV light.To ensure that the UV treated EB (UV-EB) organisms were completely inactivated,each UV-EB prep was tested on HeLa cells by indirect immunofluorescence technique.Two groups of mice were infected intranasally with low-dose of live EBs or immunized intramuscularly with high-dose of UV-EBs.First,two mice from each group were sac...