期刊论文

Hydrogen sulfide inhibits endoplasmic reticulum stress-mediated apoptosis of pulmonary fibroblasts by regulating expression of microRNA-21

中文

中国免疫学杂志

1000-484X

2018

34

11

1612-1616

10.3969/j.issn.1000-484X.2018.11.003

No.81603108:国家自然科学基金 2018JJ2339:湖南省自然科学基金 2018JJ6122:湖南省科卫联合项目 XJK17BGD070:湖南省教育科学规划课题

本校为第一机构

目的:探讨H2S是否可调节mi R-21表达抑制内质网应激介导肺成纤维细胞凋亡。方法:体外培养小鼠成纤维细胞（NIH3T3）,随机分为对照组、TGF-β1组和Na HS干预组。采用CCK-8分别检测细胞增殖,流式细胞仪检测NIH3T3凋亡率; q RT-PCR法和Western blot分别分析葡萄糖调节蛋白78（GRP78）、增强子结合蛋白同源蛋白（CHOP）和caspase-12的表达;检测mi R-21是否参与H2S抑制ERS介导的肺成纤维化细胞凋亡,将其随机分为3组:对照组、mi R-21激动剂（mi R-21 agomir）组和mi R-21拮抗剂（mi R-21 antagomir）组。mi R-21激动剂组和mi R-21拮抗剂组分别给予40μmol/L的Na HS预处理,再进行TGF-β1处理,检测GRP78...

Objective:To explore whether H_2S regulating the expression of miR-21 and then inhibition endoplasmic reticulum stress-mediated apoptosis of pulmonary fibroblasts.Methods:Mice pulmonary fibroblasts NIH3T3 were cultured in vitro.Then the cells were divided into a control group,a TGF-β1 group,and an H_2S protection group.The rate of cell proliferation was monitored by CCK-8,and the apoptosis rate of the pulmonary fibroblasts by flow cytometry.The mRNA and protein expressions of GRP78,CHOP and caspase-12 were monitored by real-time RT-PCR and Wes...