期刊论文

Mycoplasma pneumoniae capsular polysaccharides inhibit phagocytosis and membrane molecules expression of dendritic cells

中文

中国免疫学杂志

1000-484X

2018

34

1

15-18,24

10.3969/j.issn.1000-484X.2018.01.003

国家自然科学基金项目（81072418/H1005和81441065/H1905）资助；

本校为第一机构

目的:了解肺炎支原体(Mp)荚膜多糖(CPS)通过结合树突状细胞特异性细胞间黏附分子-3-结合非整合素分子(DC-SIGN)对树突状细胞吞噬功能及细胞表面膜分子表达的影响。方法:复苏培养Mp菌株,抽提和纯化CPS。培养人外周血单个核细胞来源的DC,吉姆萨染色观察和流式细胞术(FCM)鉴定;用CPS刺激DC,FCM检测DC对FITC-多聚糖的吞噬指数、DC表面特异标志CD83、HLA-DR抗原以及协同刺激分子CD80和CD86的表达。结果:培养7 d的DC有典型的树突状结构,并高表达CD11c分子。经CPS刺激后,DC内FITC-多聚糖的平均荧光强度较对照PBS处理组显著增加(P

Objective： To study the influences of Mycoplasma penumoniae capsular polysaccharide（ CPS） on the phagocytosis and membrane molecules expression of the human peripheral blood mononuclear cells derived dendritic cells by binding to dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin（ DC-SIGN）,so as to know the effect of CPS on the maturation of dendritic cells. Methods： M. pneumoniae strain was cultivated and CPS was extracted. Human peripheral blood mononuclear cells were separated and induced to dendritic cells,...