期刊论文

Effect of oxidate stress on systemic lupus erythematosus mice by transfection of prokaryotic pprI gene

中文

中国免疫学杂志

1000-484X

2018

34

9

1293-1297

10.3969/j.issn.1000-484X.2018.09.004

本文为江西卫生计生委科技计划项目(20141693)。

本校为第一机构

目的:通过检测耐辐射奇球菌ppr I基因活体转染系统性红斑狼疮（SLE）小鼠的氧化应激和疾病活动性指标,探讨其对SLE小鼠氧化应激状态的影响。方法:62只纯品系B6. MRL-Faslpr NJU雌性小鼠随机分成3组,未转染组（20只）,pEGFP-c1空载体转染组（20只）,pEGFP-c1-pprⅠ转染组（22只）,纯品系C57BL/6健康小鼠作为健康对照组（20只）。应用活体电转染技术将pEGFP-c1空载体及pEGFP-c1-pprⅠ基因重组质粒转入SLE小鼠股前肌肉。Western blot鉴定转染后SLE小鼠外周血pEGFP-c1和pEGFP-c1-pprⅠ融合基因表达。采用化学比色法检测各组小鼠外周血氧化应激标志物,ELISA法检测抗ds DNA抗体,免疫比浊法检测补体C4和...

Objective:To explore the effect on oxidative stress state by detecting oxidative stress and disease activity index of Deinococcus radiodurans transfected systemic lupus erythematosus (SLE) mice. Methods:62 pure B6. MRL-FaslprNJU female mice were randomly divided into three groups,the non transfected group,the pEGFP-c1 empty vector transfected and pEGFP-c1-pprI recombinant plasmid transfected group,the numbers of cases are 20,20 and 22 respectively. moreover,20 pure line C57BL/6 healthy mice were collected as the healthy control group. The pEGFP...