期刊论文

中文

中国免疫学杂志

1000-484X

2011

27

7

597-601

10.3969/j.issn.1000-484X.2011.07.005

本文为国家自然科学基金（30901436）及吉林省科技厅青年基金（20080162）资助

本校为第一机构

目的:观察c-Flip基因的表达变化在肝癌细胞对TRAIL诱导凋亡耐受中的作用。方法:以肝癌细胞HepG2、 Hep3B为研究对象,制备c-FlipsiRNA的细胞模型,应用流式细胞仪分析两种细胞模型对TRAIL作用后细胞周期的变化,Western blot检测其在c-Flip基因沉默前后凋亡信号传导蛋白Caspase-8、Caspase-3等的表达变化。结果:转染c-FlipsiRNA后,细胞恢复了对TRAIL诱导凋亡的敏感性,当TRAIL浓度为100 ng/ml时,HepG2及Hep3B转染组的细胞生存率为57.34%±6.13%和 43·98%±4.54%,显著低于对照组的96.44%±5.43%和101.85%±6·41%(P

Objective：To discuss the effect of c-Flip gene expression in TRAIL-induced apoptosis tolerance in hepatocellular carcinoma cell.Methods：We set hepatocellular carcinoma cell HepG2 and Hep3B as targets and prepared cells model of c-FlipsiRNA,cell cycle variation of the two types of cell models were analysed by Flowcytometry after being treated by TRAIL,the expression of signal transduction protein Caspase-8,Caspase-3,DFF-45 were detected by Western blot before and after TIP gene silencing.Results：The sensitivity of HepG2 and Hep3B to TRAIL was...