期刊论文

中文

中国免疫学杂志

1000-484X

2011

27

3

251-255,260

10.3969/j.issn.1000-484X.2011.03.014

06JJ2093:湖南省自然科学基金 08B067:湖南省教育厅青年项目 2010]212:湖南省高校"病原体致病机制与防治"科技创新团队资助项目

本校为第一机构

目的:本研究初步探讨幽门螺杆菌(H.pylori)空泡毒素单一毒力决定簇对GES-1胃黏膜上皮细胞凋亡的影响,为进一步研究H.pylori的致病机制奠定基础. 方法:将本课题组已构建的pDsRed-Monomer-C1/VacAN端真核表达载体转染至 GES-1细胞中, Western blot鉴定VacA蛋白在细胞中的表达;电子显微镜和中性红摄取试验检测GES-1细胞的空泡样变;Hoechst 33342染色、Annexin V-FITC凋亡检测试剂盒以及电镜观察细胞凋亡情况,同时采用分光光度法检测细胞Caspase-9、Caspase-3的活化程度, Rho123检测细胞跨膜电位的变化,ELISA法检测细胞色素C的释放. 结果:重组质粒pDsRed-Monomer-C1/VacA转染 GES-1细胞24小时后,电镜...

Objective：This study was designated to investigate the vacuolating activity of N-terminus of vacA protein from Helicobacter pylori and the role of mitochondrial pathway in VacA-induced apoptosis in GES-1 cells.Methods：The recombinant eukaryotic expressing vector pDsRed-Monomer-C1/VacA encoding N-terminus of Helicobacter pylori VacA was constructed by our group previously.The plasmid was transfected into GES-1 cells,and the vacuoles formation was assessed by electron microscope and neutral red uptake.Apoptosis was observed by Hoechst 33342 and...