期刊论文

Effects of lncRNA PVT1 on LPS-induced apoptosis and asthmarelated inflammatory factors secretion in bronchial epithelial cells by targeting miR-423-5p

中文

免疫学杂志

1000-8861

2021

37

12

1086-1093

10.13431/j.cnki.immunol.j.20210153

湖南省卫生健康委科研计划课题（C2019099）；

本校为第一机构

目的 探讨长链非编码RNA(lncRNA)浆细胞瘤转化迁移基因1(PVT1)靶向miR-423-5p对脂多糖(LPS)诱导的支气管上皮细胞(16HBE)凋亡和哮喘相关炎症因子分泌的影响.方法 采用1 mg/L的LPS刺激16HBE细胞12h构建体外炎症模型.实时定量PCR(RT-qPCR)分析模型细胞、哮喘患者血清中PVT1和miR-423-5p表达量.流式细胞术检测细胞凋亡.酶联免疫吸附实验检测IL-4、IL-5、IL-13分泌量.双荧光素酶报告实验和RT-qPCR分析PVT1和miR-423-5p的靶向关系.采用上述方法检测沉默PVT1或过表达miR-423-5p对LPS诱导的16HBE凋亡和哮喘相关炎症因子分泌的影响.结果 哮喘患者血清中PVT1表达水平显著升高,miR-423-5p表达水平显著降低...

The aim of this study was to investigate the effect of long non-coding RNA (lncRNA) plasmacytoma variant translocation 1 gene (PVT1) targeting miR-423-5p on lipopolysaccharide (LPS)-induced bronchial epithelial cell (16HBE) apoptosis and asthma-related inflammatory factors secretion. 16HBE cells were treated with 1 mg/L LPS for 12 hours to construct an in vitro inflammation model. Real-time quantitative PCR (RT-qPCR) was used to analyze the expression levels of PVT1 and miR-423-5p in model cells and serum of patients with asthma; flow cytometry was used to detect cell apoptosis; enzyme-linked ...