期刊论文

中文

免疫学杂志

1000-8861

2016

32

9

762-767

10.13431/j.cnki.immunol.j.20160149

湖南省自然科学基金（2016JJ2113,14JJ3104） 湖南省科技计划（2014TT2012） 南华大学博士启动基金（2014XQD40）

本校为其他机构

目的探讨TNF-α在李斯特菌(Listeria monocytogenes, LM)感染模型中对CD8~+T细胞应答的影响。方法采用尾静脉注射重组OVA的李斯特菌株感染TNF-α基因敲除(TNF-α KO)小鼠和野生型(WT)对照小鼠建立系统性感染模型。运用流式细胞术检测不同时相点TNF-α KO小鼠和WT小鼠中内源性和外源性特异性CD8~+T细胞应答反应情况。采用体内细胞杀伤实验和检测细菌滴度的方法评估TNF-α KO小鼠和WT小鼠感染后记忆期CD8~+T细胞的效应功能。接着分析记忆期和增殖期CD8~+T细胞的KLRG-1和CD127等分子的表达变化。结果与WT对照小鼠相比,TNF-α KO小鼠中CD8~+T细胞应答减少,记忆细胞增多;TNF-α KO小鼠中记忆CD8~+T细...

To reveal the role of TNF-α in regulating the CD8~+T cell response in Listeria monocytogenes (LM) infection model, we immunized TNF-α knockout (KO) mice and wide type (WT) mice with OVA recombinant LM OVA, and determined the dynamic change of the OVA-specific CD8~+ T cell response post infection. We found that the primary response in TNF-α KO mice was lower than that of WT mice, while CD8~+ T cell contraction in TNF-α KO mice decreased slower than that of WT mice, and more memory cells were generated in TNF-α KO mice compared with WT mice....