期刊论文

中文

免疫学杂志

1000-8861

2016

32

8

661-665

10.13431/j.cnki.immunol.j.20160129

国家自然科学基金（81570703）； 湖南省自然科学基金（2016JJ2113,14JJ3104）； 南华大学博士启动基金（2014XQD40）；

本校为其他机构

目的探讨LIGHT对利什曼原虫(L. major)感染模型中CD4~+T细胞亚群免疫应答的影响。方法采用LIGHT KO和野生型(WT)小鼠,经小鼠脚掌注射L. major建立感染模型。从感染第2周至24周连续观察被感染脚掌的病损程度,并测定感染24周小鼠体内寄生虫载量。其次,取感染第7天小鼠引流淋巴结(dLN)细胞,运用3H-TdR掺入方法检测T细胞体外刺激的增殖情况,并且利用ELSIA检测体外刺激后dLN细胞IL-12和IFN-γ的表达水平。最后,通过流式细胞术检测感染第7天小鼠脾脏和dLN中Treg水平。结果LIGHT KO小鼠被感染脚掌和dLN的寄生虫载量均显著高于WT对照小鼠。感染早期LIGHT KO小鼠dLN中T细胞增殖水平,以及IL-12和IFN-γ的表...

Helper T cell subsets play a very important role in the resistance or susceptibility to infection of Leishmania major (L. major). Though there were reports finding that LIGHT can influence Th1 response after L.major infection, the role of LIGHT in regulating helper T cell subsets in L. major infection model are not fully understood. In this report, we aimed to investigate the effect of LIGHT on CD4~+ cell responds in L. major infection model. L. major was injected to footpad of mice to build an infection model. Then we found that LIGHT KO mice ...