期刊论文

中文

免疫学杂志

1000-8861

2015

31

12

1059-1062

10.13431/j.cnki.immunol.j.20150223

国家自然科学基金（81273322） 湖南省教育厅资助科研项目（13C876） 郴州市科技局资助科研项目（2012CJ126）

本校为第一机构

目的预测和鉴定梅毒螺旋体(Treponema pallidum, Tp)Tp92蛋白的Th细胞表位,为深入探讨这些表位在梅毒表位疫苗中的作用奠定基础。方法采用RANKPEP和SYFPEITHI软件分析工具联合预测Tp92蛋白的Th细胞表位,人工合成5条表位多肽,以5条表位多肽(同时设ConA阳性对照和RPMI-1640阴性对照)分别刺激梅毒患者和正常人外周血单个核细胞(PBMC),用CCK-8法鉴定预测的Th细胞表位,进一步以ELISA检测INF-γ和IL-4的分泌水平,区分Th细胞表位的类型。结果软件预测显示,Tp92蛋白的P1(103-118AA)、P2(694-712AA)、P3 (668-680AA)、P4 (300-313AA)、P5 (396-410AA)氨基酸序列最可能为其Th细胞表位。CCK-8法检测显示,P2...

To predict and identify the Th-cell epitopes of Treponema pallidum（Tp） Tp92 protein and provide a basis for further discussion on roles of these epitopes in epitope-based syphilis vaccine design, the Th cell epitopes of the protein Tp92 were analyzed and predicted with RANKPEP and SYFPEITHI software, and 5 peptides containing predicted epitopes were artificially synthesized. The five predicted peptides of Tp92 were in vitro co-incubated with peripheral blood mononuclear cells（PBMCs） isolated from syphilis patients and normal humans respecti...