期刊论文

中文

免疫学杂志

1000-8861

2013

29

2

99-103

10.13431/j.cnki.immunol.j.20130023

国家自然科学基金(30901352) 国家自然科学基金(81001318) 传染病预防控制国家重点实验室自主研究开放课题(2012SKLID308) 湖南省高校科技创新团队(湘教通[2010]53号) 湖南省研究生创新基地资助项目

本校为第一机构

目的探讨肺炎嗜衣原体(Chlamydophila pneumoniae,Cpn)热休克蛋白(heat shock protein,HSP)10(CHSP10)诱导人单核细胞分泌炎症因子的作用及Toll样受体(Toll-like receptor,TLR)2、TLR4与此作用的相关性。方法以去内毒素活性的不同质量浓度CHSP10(0.5、1、5、10、20、30μg/ml)刺激THP-1细胞0、6、12、24、36、48、60 h,检测蛋白未处理组、加热处理组、去蛋白处理组中IL-1β及IL-6水平;用CHSP10刺激C3H系野生型(C3H/HeN)和TLR4缺陷型(C3H/HeJ)小鼠腹腔巨噬细胞,分别检测IL-1β及IL-6水平;用CHSP10刺激被抗TLR2/TLR4抗体处理的THP-1细胞,检测IL-1β、IL-6的变化。结果CHSP10可诱导THP-1细胞产生炎...

To investigate the role and mechanism of heat shock protein 10(HSP10) of Chlamydophila pneumoniae in inducing inflammatory reaction.Purified native recombinant HSP10 from C.pneumoniae(CHSP10) were inactivated for cleaning contaminated endotoxin and were used to stimulate THP-1 with different concentrations for different time,then the cytokines levels were measured by using human enzyme-linked immunosorbent assay kit.Peritoneal macrophages from TLR4-deficient mice and wide-type mice were stimulated with endotoxin-free proteins respectively,and t...