期刊论文

中文

免疫学杂志

1000-8861

2012

28

3

203-207

10.13431/j.cnki.immunol.j.20120046

国家自然科学基金（81102230） 湖南省高校科技创新团队（湘教通[2010]53号）

本校为第一机构

目的分析沙眼衣原体蛋白水解酶CT841在感染细胞中的定位并探讨其抗原性。方法利用PCR技术获得衣原体CT841基因,将基因序列克隆到载体pGEX6p,转化大肠杆菌XL1-blue,IPTG诱导表达融合蛋白GST-CT841。融合蛋白经纯化后免疫小鼠制备特异性抗体,间接免疫荧光法分析CT841在感染细胞中的分布特征;ELISA法分析CT841的抗原性。结果CT841原核表达重组体成功构建;CT841在感染细胞的表达模式与主要外膜蛋白MOMP相似,而与衣原体蛋白酶样活性因子CPAF及包涵体膜蛋白IncA的分布模式不同;CT841与衣原体感染患者、猴、鼠血清均发生强烈的免疫反应。结论沙眼衣原体蛋白酶CT841是定位于衣原体菌体上的免疫优势抗原。...

This study aims to localize protease CT841 in Chlamydia trachomatis-infected cells and investigate the antigenicity of the protein.Firstly,Chlamydia trachomatis CT841 gene was amplified by PCR and insert into pGEX-6p vector,and then the constructed vector was transformed into E.coli XL1Blue and induced by IPTG.After purified with Glutathione Sepharose 4B beads,the CT841 fusion protein was used to immunize mice to produce polyclone antibody,which were subsequently used to localize the endogenous CT841 protein by indirect immunofluorescence assay...