This study is aimed to explore the effects of IFN-γ and TNF-α on the apoptosis of insulinoma cells line MIN6.The cytotoxicity of IFN-γ,TNF-α,or IFN-γ/TNF-α synergism to MIN6 cells was assessed by MTT,and further confirmed morphologically by microscopy and laser confocal microscopy.The apoptotic and necrotic effects of IFN-γ and TNF-α on MIN6 cells were differentiated by FACS followed with Annexin V-FITC/7-aminoactinomycin D（7-AAD） double staining.In contrast to IFN-γ or TNF-α alone,IFN-γ/TNF-α synergism had an obvious inhibition i...
