期刊论文

中文

免疫学杂志

1000-8861

2010

26

11

982-986

10.13431/j.cnki.immunol.j.20100235

国家自然科学基金（30771931） 湖南省教育厅科研基金（08C745）

本校为第一机构

目的表达LTB-NspA重组融合蛋白,鼻饲免疫雌性Balb/c小鼠,分析重组融合蛋白的免疫活性,为研制抗淋球菌蛋白疫苗提供实验依据.方法转化并鉴定pET30a/ltB-nsp A原核重组质粒后,在E.coli BL21中表达重组融合蛋白.鼻饲途径免疫雌性Balb/c小鼠,检测NspA特异性体液免疫和细胞免疫水平.结果 LTB-NspA融合蛋白组生殖道黏膜产生的NspA特异性sIgA水平和血清IgG水平随免疫时间呈上升趋势,第6周sIgAA450nm达0.689,明显高于NspA组和LTB,蛋白溶解液(solution buffer)对照组(P

The aim of the study is to express LTB-NspA fusion protein and identify immunocompetence of the protein,and thus provide excremental basis for preparing anti-gonorrhoeae protein vaccine.The prokaryotic recombinant plasmid pET-30a/ltB-nspA was extracted,and then identified by PCR and the double-enzymes digestion,finally transformed into E.coli BL21 for protein expression.We analyzed the immunocompetence（humoral immunologic response and the cellular immunologic response） of the recombinant fusion protein in female Balb/c mice through intranasal...