期刊论文

Relationship between cognitive impairment induced by local radiation and morphological changes of hippocampal microglia

Li, C.;He, J.

中文

辐射研究与辐射工艺学报

1000-3436

2021

39

6

63-71

10.11889/j.1000-3436.2021.rrj.39.060301

基金资助：湖南省自然科学基金项目（ 2019 JJ40257、 2019 JJ40242、 2019 JJ50504 ）资助 第一作者：张圆，女，1997 年3月出生，现为南华大学衡阳医学院病理学专业在读硕士研究生 ，研究方向为神经生物学 通信作者：李彩，硕士，讲师，E-mail: 375933697@qq.com；何洁，副教授，硕士生导师，E-mail: hejie430@126.com 收稿日期：初稿 2021-04-06；修回 2021-05-18 Supported by the Natural Science Foundation of Hunan Province (2019JJ40257, 2019JJ40242, 2019JJ50504) First author: ZHANG Y uan (female) was born in March 1997. Now she is a graduate student at the Department of Pathology of UniversityofSouthChina,majoringinneurobiology Corresponding author: LI Cai, master's degree, lecturer , E-mail: 375933697@qq.com;HE Jie, associate professor , master's supervisor , E-mail: hejie430@126.com Received06April2021;accepted18May2021

本校为第一机构

探讨小胶质细胞在头部X射线照射所致认知障碍过程中的作用。18只ICR(Institute of Cancer Research)小鼠随机分为对照组和头部辐照组,头部辐照组小鼠接受X射线单次头部辐照至吸收剂量10 Gy。辐照56 d后分别采用新旧事物识别实验和新旧位置识别实验检测小鼠认知功能;免疫组织化学法检测小鼠海马小胶质细胞标记物IBA-1阳性细胞数目变化;Sholl Analysis定量分析小胶质细胞形态变化;RT-PCR检测海马M1/M2型小胶质细胞标记物(CD16、INOS、CD68、CXCL10、Arg1、CD206)mRNA水平表达变化。结果显示:与对照组相比,头部辐照组小鼠新事物时间辨别指数(p

To investigate the role of microglia in the process of cognitive impairment caused by local ionizing radiation, eighteen male mice from the institute of cancer research (ICR) were randomly divided into a control group (Control) and a head 10 Gy irradiation group (Irradiation, 10 Gy-IR). The head ionization irradiation group underwent X-ray (Siemens linear accelerator) single head irradiation illuminated to an absorbed dose of 10 Gy. Fifty-six days later, the new and old object recognition test and the new and old place recognition test were use...