期刊论文

Effects of gamma-ray irradiation on cognitive function and neuronal death in mice

中文

辐射研究与辐射工艺学报

1000-3436

2018

36

5

13-18

10.11889/j.1000-3436.2018.rrj.36.050202

2018JJ3460:湖南省自然科学基金 2018JJ4034:湖南省自然科学基金 14A125:教育厅项目 17B230:教育厅项目 2015XQD27:南华大学博士科研启动基金 2015XQD28:南华大学博士科研启动基金 :湖南省研究生科研创新项目

本校为第一机构

57只2月龄雌性昆明小鼠分别接受不同剂量~(137)Cs γ射线(0、4、6、8、10 Gy)单次全身照射,观察小鼠生存率及体重变化情况,35 d后进行旷场实验、新旧位置识别实验行为学检测,并采用Western blot方法检测小鼠海马区神经元标记物NeuN的表达。结果表明:4 Gy剂量组小鼠生存率100%,且一般健康状况较好,而给予8 Gy及10 Gy吸收剂量时,小鼠死亡率明显增加;与对照组相比,4 Gy及6 Gy剂量组小鼠在新旧位置识别实验中,对新位置的时间辨别指数百分比均明显降低(36.04% ± 4.39% vs. 11.45% ± 6.34% vs. -1.40% ± 9.27%,p

Fifty-seven female Kunming mice aged 2 months received full-body irradiation once with ~(137)Cs γ-rays at doses of 0, 4, 6, 8, and 10 Gy. The survival rate and body weight were then observed for 35 d. The animals' behaviors were analyzed using open-field and novelty location tests 35 d post irradiation. The expression of the neuronal marker NeuN in hippocampus was determined using western blot. The results showed that the mice in the 4 Gy group exhibited a higher survival rate and superior general status than did those in the other groups. Com...