期刊论文

Expression of FOXM1 and its effects on radiation sensitivity in esophageal squamous cell carcinoma (ESCC)

中文

辐射研究与辐射工艺学报

1000-3436

2016

34

6

060202-1-060202-8

10.11889/j.1000-3436.2016.rrj.34.060202

湖南省自然科学基金项目（2016JJ2115）； 南华大学博士启动基金（2015XQD31）； 湖南省卫生计生委科研计划课题项目（C2015-17）资助~~；

本校为第一机构

采用Real-time PCR检测40例食管鳞状细胞癌及相对应癌旁组织中FOXM1(Forkhead box M1)mRNA表达情况;免疫组织化学方法检测94例食管鳞状细胞癌组织中FOXM1蛋白的表达情况,分析其与鳞状细胞癌临床病理之间的关系;采用针对FOXM1的siRNA沉默ECA-109、TE-1细胞中FOXM1基因表达水平,通过免疫荧光染色、克隆形成实验检测FOXM1对食管鳞状细胞癌放射敏感性的影响。结果表明,FOXM1 mRNA表达水平在食管癌组织中显著高于癌旁组织(p

Real-time PCR was used to examine the expression of FOXM1 (Forkhead box M1) in 40 esophageal squamous cell carcinoma (ESCC) tissues, and the adjacent normal tissues. Immunohistochemistry was used to examine the expression of FOXM1 protein in 94 ESCC tissues, and to analyze the relationship between ESCC and clinic pathologic. A small interfering RNA targeting FOXM1 was designed to silence the endogenous FOXM1 expression of ECA-109 and TE-1 cells. The effect of FOXM1 on radiation sensitive in esophageal squamous cell was observed by immunofluores...