期刊论文

中文

辐射研究与辐射工艺学报

1000-3436

2015

33

6

060202-1-060202-6

10.11889/j.1000-3436.2015.rrj.33.060202

(81272994):国家自然科学基金项目 (C2015-17):湖南省卫生计生委科研计划课题项目 (13C805):湖南省教育厅科学研究项目 (2014XCX24)资助Supported by the National Natural Science Foundation of China(81272994):南华大学研究生科研创新项目 (C2015-17):Health and Family Planning Commission Scientific Research of Hunan Province (13C805):Education Department Scientific Research of Hunan Province (2014XCX24):Graduate Student Research Innovation Project of University of South China

本校为第一机构

以γ-射线辐照L-02细胞建立辐射损伤模型,用不同浓度的黄芪甲苷对其进行处理。通过MTT法检测细胞存活率;克隆形成实验观察黄芪甲苷对辐照后L-02细胞克隆形成的影响;生长曲线观察黄芪甲苷对L-02细胞的增殖能力的影响;WST-1法检测总超氧化物歧化酶（SOD）活力;微板法检测还原型谷胱甘肽（GSH）活力及丙二醛（MDA）含量;DCFH-DA法检测活性氧（ROS）含量。结果表明:终浓度为80μg/m L以下的黄芪甲苷对L-02细胞增殖无影响;辐照后细胞存活率随受照剂量增大而降低,随辐照后时间增长而降低;黄芪甲苷能够提高辐射损伤后L-02细胞的增殖能力,抑制辐射所致的胞内ROS和MDA含量升高,同时使胞内SOD和GSH活力有所...

ABSTRACEL-02 cells were treated with astragaloside IV of different concentrations, and irradiated withγ-rays to establish the radiation damage models. MTT method was used to detect cell viability. Clone formation assay and growth curves were used to observe the effects of astragaloside IV on clone formation and proliferative capability. WST-1 method, microplate test, and DCFH-DA method were used to detect SOD activity, GSH activity, MDA content, and ROS content, respectively. The results showed that astragaloside IV had no effect on the prolif...