Identification by cDNA cloning of abundant sRNAs in a human-avirulent Yersinia pestis strain grown under five different growth conditions

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成果类型：

期刊论文

作者：

Qu, Yi;Bi, Lijun;Ji, Xiaolan;Deng, Zhongliang;Zhang, Hongtai;...

通讯作者：

Han, Yanping

作者机构：

[Yan, Yanfeng; Qu, Yi; Han, Yanping; Yang, Ruifu] Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China.

[Bi, Lijun; Li, Aqian; Zhang, Hongtai; Wang, Ming] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100080, Peoples R China.

[Bi, Lijun; Li, Aqian; Zhang, Hongtai; Wang, Ming] Chinese Acad Sci, Inst Phys, Key Lab Noncoding RNA, Beijing 100080, Peoples R China.

[Ji, Xiaolan; Huang, Xinxiang] Jiangsu Univ, Dept Biochem & Mol Biol, Zhengzhou, Jiangsu, Peoples R China.

[Deng, Zhongliang] Univ S China, Sch Publ Hlth, Dept Sanit Inspect, Hengyang, Peoples R China.

通讯机构：

[Han, Yanping] B

Beijing Inst Microbiol & Epidemiol, State Key Lab Pathogen & Biosecur, Beijing, Peoples R China.

语种：

英文

关键词：

cDNA library construction;small RNA;Yersinia pestis

期刊：

FUTURE MICROBIOLOGY

ISSN：

1746-0913

年：

2012

卷：

期：

页码：

535-547

DOI：

10.2217/fmb.12.13

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [30971629, 31171248, 30930001]

机构署名：

本校为其他机构

院系归属：

公共卫生学院

摘要：

Aims: sRNA regulation is supposedly involved in the stress response of a pathogen during infection. Yersinia pestis, the etiologic agent of plague, must encounter temperature and microenvironment changes, given its lifestyle. Here, we used the cDNA cloning approach to discover full-length sRNA candidates that are highly expressed in Y. pestis under five different growth conditions. Materials & methods: The cDNA cloning approach was improved by combining the traditional cDNA library construction with the prevalent rapid amplification of cDNA end...

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Identification by cDNA cloning of abundant sRNAs in a human-avirulent Yersinia pestis strain grown under five different growth conditions

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