期刊论文

中文

广东医学

1001-9448

2016

37

15

2238-2241

10.13820/j.cnki.gdyx.2016.15.004

湖南省科技厅基金资助项目（编号:2015JC3088）；

本校为第一机构

目的：探讨黄芩素对人前列腺癌PC－3细胞增殖、侵袭的影响及其相关机制。方法培养人前列腺癌PC－3细胞，分别予20μL的培养液（对照组）、无水乙醇（溶媒组）、黄芩素溶液（100、200μmol／L黄芩素组）处理细胞48 h后，通过四甲基偶氮唑蓝（ MTT）法分析PC－3细胞的增殖情况，Transwell侵袭实验检测PC－3细胞的侵袭能力，荧光实时定量PCR检测PC－3细胞中Ezrin mRNA表达，Western blot测定PC－3细胞中Ezrin、Bcl－2、Bax蛋白表达，原位末端标记法（ TUNEL）检测PC－3细胞凋亡率。结果100、200μmol／L黄芩素作用PC－3细胞48 h后，细胞增殖抑制率、凋亡率以及Bax蛋白表达水平增加，平均穿膜细胞数...

Objective To investigate the effects of baicalein on the proliferation and invasion of human prostate cancer PC- 3 cells and its molecular mechanisms. Methods Human prostate cancer PC- 3 cells were cultured in vitro,and inoculated with 20 μL culture solution（ control group）,dehydrated alcohol（ vehicle group）,and baicalein solution（ 100 and 200 μmol/L） for 48 h,respectively. The MTT method was used to analyze the proliferative ability of PC- 3cells. The cellular invasion was measured by using Trans- well invasion experiment. Ezrin mRNA e...