期刊论文

Effects and Mechanisms of AXL on the Invasive Ability of Epithelial Ovarian Carcinoma Cell Lines

中文

肿瘤药学

2095-1264

2012

2

3

177-179+183

10.3969/j.issn.2095-1264.2012.03.005

湖南省科技厅科技计划资助项目（编号:2010FJ3078）；

本校为第一机构

目的 探讨AXL 表达水平与上皮性卵巢癌细胞侵袭性的关系及其机制.方法 使用OVCAR3 细胞为研究载体,通过siRNA 干扰细胞中AXL 的表达,通过RT-PCR 检测两组细胞中AXL 以及MMP-9 基因表达水平,Transwell 法比较干扰组细胞与对照组细胞侵袭能力,并比较siRNA 干扰组细胞与对照组细胞在裸鼠体内的成瘤能力.结果 干扰组OVCAR3 细胞中AXL 以及基质金属蛋白酶-9 表达水平明显降低,差异有统计学意义(P

Objective To explore the effects and mechanisms of AXL on the invasive ability of epithelial ovarian carcinoma cell lines. Methods siRNA was used to inhibit the expression of AXL in OVCAR3, taken as the experiment group. The expression of MMP-9 and AXL were detected by RT-PCR, and the invasive ability of OVCAR3 was detected by Ttranswell method. The transplanted tumor number and weight were compared between the experiment group and control group. Results The expression level of AXL and MMP-9 were both significantly lower in the experiment group...