De novo synthesis of PCR templates for the development of SARS diagnostic assay

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成果类型：

期刊论文

作者：

Zhang, L;Meng, B;Liao, DF;Zhou, LY;Zhang, X;...

通讯作者：

Li, K

作者机构：

[Jia Zhang; Zhang, X; Chen, LL; Guo, ZF; Peng, CY] Genomapping Inc., Tianjin

[Meng, B; Zhou, LY; Hu, YH] Shanghai Institute of Brain Functional Genomics, East China Normal University, Shanghai

[Liao, DF; Zhu, BY] Institute of SNP, Institute of Pharmacy and Pharmacology, National University, Hengyang

[Lee, PP] University of California in San Diego, San Diego, USA

[Xu, XM] Department of Medical Genetics, The First Military Medical University, Guangzhou

通讯机构：

[Li, K ]

Genomapping Inc, Tianjin, Peoples R China.

语种：

英文

关键词：

gene synthesis;SARS;PCR;sequential primer extension

期刊：

Molecular Biotechnology

ISSN：

1073-6085

年：

2003

卷：

期：

页码：

107-112

DOI：

10.1385/MB:25:2:107

机构署名：

本校为其他机构

院系归属：

药学与生物科学学院

摘要：

A novel coronavirus was identified as the cause for severe acute respiratory syndrome (SARS). The complete sequence of SARS genome has provided an opportunity for the development of molecular diagnostic assays. To restrain further outbreak of SARS, the World Health Organization has posted several pairs of polymerase chain reaction (PCR) primers for early diagnosis and urged more research to be done on PCR protocols. Here we report a strategy for the de novo synthesis of PCR templates complimentary to the SARS virus genome, which has the advantage of working on PCR templates without concern abo...

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De novo synthesis of PCR templates for the development of SARS diagnostic assay

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