期刊论文

Significance of Sirtuin-3 protein in invasion and metastasis of breast cancer

中文

中国临床药理学杂志

1001-6821

2021

37

22

3051-3055

10.13699/j.cnki.1001-6821.2021.22.009

湖南省自然科学基金资助项目（2018JJ2354）；

本校为第一机构

目的分析乳腺癌患者肿瘤组织中Sirtuin 3(SIRT3)蛋白的表达情况及其临床病理特征,研究SIRT3在乳腺癌上皮间质转化(Epithelial-mesenchymal transformation,EMT)中的作用及机制。方法收集我院确诊为乳腺癌的180例女性患者的临床资料,通过免疫组化法检测180例乳腺癌组织及60例乳腺增生组织中SIRT3蛋白的表达。以蛋白质印迹(Western blot)法筛选出SIRT3低表达乳腺癌细胞系,并选择MCF -7乳腺癌细胞做后期细胞实验细胞系。转染空质粒作为对照组,转染SIRT3过表达质粒为实验组,观察乳腺癌细胞增殖、侵袭、转移及上皮间质转化情况。结果SIRT3蛋白在乳腺癌组织及乳腺增生组织中阳性率分别为37.8%(68例/18...

Objective To analyze the expression of Sirtuin 3(SIRT3) in breast cancer tissues and its clinicopathological characteristics,to study the effect and mechanism of SIRT3 in epithelial-mesenchymal transformation (EMT) of breast cancer.Methods The clinical data of 180 female patients with breast cancer and 60 cases of breast hyperplasia were collected,and the expression of SIRT3 protein was detected by immunohistochemistry.Western Blot was used to screen breast cancer cell lines with low SIRT3 expression,and MCF -7 cell line was selected for subseq...