期刊论文

Protective effects of red clover flavonoids on rat pancreatic β cells induced by high glucose by regulating the expression of miR -99a -3p /CD36

中文

中国临床药理学杂志

1001-6821

2021

37

18

2427-2432

10.13699/j.cnki.1001-6821.2021.18.012

湖南省教育厅科学研究课题资助项目（18C0441）；

本校为第一机构

目的研究红车轴草总黄酮对高糖诱导的大鼠胰岛β细胞损伤的影响,及其潜在的分子机制。方法本实验分为对照组,模型组,低、中、高剂量实验组,miR-NC组,miR-99a-3p组,anti-miR-NC,anti-miR-99a-3p组,模型+ miR-NC组,模型+ miR-99a-3p组,模型+ si-NC组,模型+ si-CD36组,高剂量实验+ anti-miR-NC组和高剂量实验+ anti-miR-99a-3p组。对照组给予5.5 mmol·L~(-1)葡萄糖处置;模型组给予25 mmol·L~(-1)葡萄糖处置;低、中、高剂量实验组分别给予12.5,25.0和50.0 mg·L~(-1)红车轴草总黄酮和25 mmol·L~(-1)葡萄糖处置; miR-NC组、miR-99a-3p组、anti-miR-NC组、 anti-miR-99a-3p组分别转染miR-NC、miR-9...

Objective To investigate the effects of red clover flavonoids on the injury of rat pancreatic β cells induced by high glucose and its potential molecular mechanism. Methods This experiment was divided into control group,model group,experimental-L,-M,-H groups,miR -NC group,miR-99a-3p group,anti-miR-NC,anti-miR-99a-3p group,model + miR-NC group,model + miR-99a-3p group,model + si-NC group,model + si-CD36 group,experimental-H + anti-miR-NC group and experimental-H + anti-miR-99a-3p group. The pancreatic islet β cells in the control group were t...