期刊论文

中文

肿瘤防治研究

1000-8578

2016

43

11

926-932

10.3971/j.issn.1000-8578.2016.11.002

81374013:国家自然科学基金 81102854:国家自然科学基金 B2015-182:湖南省卫生计生委科研项目

本校为第一机构

目的观察RORα高表达对MGC803细胞增殖与迁移侵袭的影响。方法构建高表达RORα人胃癌MGC803细胞。Real-time PCR或RT-PCR和Western blot检测RORα、MMP-9与TIMP3 mRNA和蛋白表达。MTT、流式细胞术、迁移和侵袭实验检测RORα高表达对MGC803细胞增殖、细胞周期、迁移和侵袭能力的影响。结果RORα高表达MGC803细胞RORα mRNA和蛋白表达显著上调。在48、72与96 h, RORα高表达细胞的增殖活性明显低于对照组和空载体组(P

Objective To investigate the effect of RORct overexpression on human gastric cancer MGC803 cells proliferation, migration and invasion. Methods The expressions of RORct, MMP-9 and TIMP3 mRNA and protein were detected by Real-time PCR or RT-PCR and Western blot. The effect of RORct overexpression on the proliferation, cell cycle, migration and invasion of MGC803 cells were detected by MTf, flow cytometry, wound healing and Transwell assays. Results The expressions of RORa mRNA and protein were stably increased in the RORa/MGC803 cells. The proli...