期刊论文

中文

细胞与分子免疫学杂志

1007-8738

2022

38

10

872-879

国家自然科学基金（81101274）；

本校为第一机构

目的 探究Th17细胞/调节性T细胞（Treg）失衡在日本血吸虫病肝虫卵肉芽肿形成中的作用。方法 采用6～8周龄的BALB/c小鼠建立日本血吸虫感染模型，在感染后第2、 4、 6、 8周处死小鼠，收集肝脾组织和血液。采用HE染色和Masson染色检测小鼠肝组织病变情况；流式细胞术检测肝、脾组织中Th17细胞、 Treg百分比变化；实时定量PCR检测肝组织中白细胞介素6（IL-6）、 IL-10、 IL-17、 IL-23和维甲酸相关孤核受体γt（RORγt）、叉头盒转录因子P3（FOXP3）的mRNA水平；ELISA检测血清中IL-6、 IL-17、 IL-23和转化生长因子β（TGF-β）表达水平。制备日本血吸虫可溶性虫卵抗原（SjSEA）,体外刺激小鼠脾细...

Objectives To investigate the effect of the imbalance of Th17/Treg on egg granuloma formation of liver with Schistosomiasis japonicum. Methods The BALB/c mice were infected with Schistosoma japonicum cercariae to establish a model of Schistosomiasis japonica. The blood samples, liver tissues and spleen tissue were harvested at the 2nd, 4th, 6th, 8th week, respectively. HE staining and Masson staining were performed to assess the pathological characteristics of the liver. Flow cytometry (FCM) was conducted to evaluate the proportion of CD4(+) T ...